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Applied Biotechnology and Biosensor Laboratory |
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Phone:
318-257-5280 |
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Dr.
Guilbeau leads the Applied Biotechnology and Biosensors laboratory. He is the Academic Director for Biomedical Engineering
and the Watson Eminent Scholar Chair in Biomedical Engineering. He is also the Director of the Center for
Biomedical Engineering Research and Rehabilitation Science (CBERS). Prior to joining Louisiana Tech University,
he was the Olin Professor of Bioengineering at Arizona State University
(ASU). At ASU he was the Founding
Chair of the Harrington Department of Bioengineering and also served as Chair
of ASU’s Department of Chemical, Bio & Materials Engineering. He has been named a fellow of the American
Institute of Medical and Biological Engineering and the Biomedical
Engineering Society. He is past
President of the Biomedical Engineering Society. He helped establish the National Council of
Chairs of Bioengineering and Biomedical Engineering Programs and has served
as its chair. He is a past chair of
the Academic Council of the American Institute of Medical and Biological
Engineering. Dr. Guilbeau is an ABET
Program Evaluator and represents BMES as an alternate on the ABET Board of
Directors. He served as the first
chair of the Accreditation Activities Committee of the Biomedical Engineering
Society and helped insure a smooth transition of Biomedical Engineering
accreditation activities oversight from the IEEE to the BMES. His teaching areas are mostly focused on
the application of transport phenomena in biomedical engineering and
introducing students to opportunities in biomedical engineering. His research
experience includes contributions in the areas of biosensor development, biotransport phenomena, systems analysis and simulation
of physiological systems with applications in diabetes, cardiovascular
disease and applied biotechnology. Staff
Mrs. Nestorova
provides half-time research support for all biological activities within the
Laboratory for Biosensors and Applied Technology and is a Research Scientist
for Biovations, LLC working within the Applied Biotechnology and Biosensors
Laboratory to develop thermoelectric methods for genetic mutation (SNP)
genotyping. Mrs. Nestorova has
both academic training and practical experience in molecular biology,
nanotechnology, and microfluidics.
Prior to joining LA Tech she worked at Stanford University where she
was involved in studies using gene expression, comparative genomic
hybridization (CGH), chromatin immunoprecipitation
(ChIP), methylation and microRNA assays. Her expertise includes gene expression analysis,
quantification of protein and protein isoforms, and detection and kinetic
analysis of biomolecular interactions. She has
experience using the Luminex 100 for measurement of
inflammatory marker levels in biological fluids, the Firefly 3000 for
quantification of proteins and protein isoforms, and the ForteBio
Octet system for detection and kinetic analysis of bimolecular
interactions. Current projects include
contributions to the
development of a novel thermoelectric method for DNA sequencing in a
microfluidic device, a novel method for thermoelectrically detecting DNA
hybridization, and new technology to quantify the levels of 8OHdG in
Alzheimer transgenic mice urine using capillary electrophoresis with laser
induced fluorescence detection.
Mr. Kopparthy received the Bachelor of Technology degree in
Biomedical Engineering from Jawaharial Nehru Technological
University and the Master of Science degree in Biomedical Engineering from
Louisiana Tech University. He is
currently employed by Biovations, LLC and is working in the Applied
Biotechnology and Biosensors Laboratory developing thermoelectric
microfluidic systems for genetic mutation assay. His technical skills include: Lay-by-Lay
self-assembly, MEMs fabrication, mask design and alignment, microfluidic
device design and fabrication, micro-controller programming, MATLAB signal
and image processing, CoventorWARE simulation, Keil u-vision, AutoCAD, LabVIEW,
NI LabVIEW signal Express, PSPICE, Multisim, C-programming.
His prior research experience includes the design and development of a
micro-controller based heart rate acquisition system, and development of
thermoelectric sensing systems for the measurement of glucose, L-glutamate
and DNA sequencing. Doctoral
Students
Mr.
Tangutooru’s research seeks to design, fabricate, and test the performance of
a microfluidic L-Glutamate (L-Glu) biosensor based
on a novel thermoelectric biorecognition strategy. L-Glu is an
important excitatory neurotransmitter that plays a key role in brain cell
processes such as learning and memory.
His work seeks to provide scientists with a low cost L-Glu biosensor technology for use in understanding the
spatial and temporal dynamics of brain cell and network excitation and
communication. In collaboration with
neuroscientist Dr. Mark DeCoster the research seeks to answer the question,
“Is it possible to monitor the rapid dynamics of brain cell L-Glu release and uptake by detecting the heat that is
released from deamination of L-Glu during an
enzymatic reaction?” The goal is to
develop a sensing device with the sensitivity, stability and response time
needed to study the L-Glu release from neurons,
astrocytes, and glioma. To achieve this goal, three objectives will be
accomplished. Objective 1: Design and
fabricate a microfluidic device with an integrated thermoelectric L-Glu biosensor with the sensitivity and response time
needed to measure rapidly changing L-Glu
concentrations within the small volume of fluid flowing within the
microfluidic device. Objective 2:
Immobilize brain cells on the surface of the lower channel wall of the
microfluidic device developed in Objective 1 at a location upstream from the
microfluidic device’s integrated thermoelectric L-Glu
biosensor. In a series of carefully
designed experiments, demonstrate that the dynamic release of L-Glu by the immobilized brain cells can be monitored by
the thermoelectric L-Glu biosensor. Objective
3: Use the microfluidic device with integrated L-Glu
biosensor and each of the three types of immobilized brain cells to study the
dynamics of L-Glu release from the respective cells
following stimulation of L-Glu release.
Mr. Reis earned his B.S
degrees in Biomedical and Chemical Engineering at Louisiana Tech. He is
continuing his education at Louisiana Tech by working on his M.S. in
Microsystems Engineering and his Ph.D. in Biomedical Engineering. Mr. Reis’s
research aims at designing, fabricating, and testing a non-enzymatic glucose
sensor by measuring changes in osmotic pressure. Unlike enzymatic sensors,
non-enzymatic sensors are not limited or affected by enzymatic degradation,
oxygen concentrations, and electrode fouling among other factors. This fairly
new concept of measuring changes in osmotic pressure to detect changes in
glucose levels enables the use of simple technology that is both passive and
power-conservative to continuously monitor glucose levels. Previous research
has showed that a glucose osmotic pressure sensor can operate effectively in
a static fluid. Mr. Reis’s work seeks to investigate the possibility of
operating such a device in a dynamic fluid. His work will progress towards
imitating the dynamics of blood flow in arteries and capillaries in order to
create a functioning continual glucose sensor that can directly measure blood
glucose. His work is funded by the National Science Foundation Graduate
Research Fellowship Program. Master’s Students
Ms. Bindu's research intents to design, fabricate and test the performance of a microfluidic
device to detect the DNA hybridization.
Unlike various other methods of DNA hybridization, she utilizes the
nano volts of natural heat produced by the exothermic reactions of DNA
hybridization. Her work involves the fabrication of highly sensitive
thermopiles for the thermoelectric method of detecting DNA hybridization. Her
research also includes different ways of immobilizing DNA.
Ms. Reddy is pursuing the M.S. in Molecular Science and
Nanotechnology. Her research seeks to
develop various strategies for immobilizing DNA on microfluidic devices for
microfluidic DNA sequencing. She is
also investigating novel methods to quantify DNA immobilization. Applications include microfluidic detection
of genetic mutations. |
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