1991 - BS/MS, Kiev State University, Kiev, Ukraine
1996 - Ph.D., Institute of Molecular Biology and Genetics, Kiev, Ukraine
1997 - 2003 - Postdoctoral training, University of Texas at Austin and European
Molecular Biology Laboratory, Heidelberg, Germany
Gene editing and genome engineering
There are three directions of our current research: gene editing and genome engineering, protein engineering, and aging.
Our main research direction is gene editing aimed to repair genetic defects in individuals with genetic diseases. Currently, we are working on correcting mutations that cause sickle cell anemia disease. In our experiments we use several gene editing/genome engineering tools: CRISPR/Cas9 and TALENs systems along with the target-specific variants of site-specific DNA recombinases: Flp, Cre, and related recombinases. For our genome engineering experiments, we design non-viral vectors with different functional elements, including S/MAR and insulator elements. We also experiment with in vitro transcribed mRNA, shRNA, transposase-mediated integrating vectors, and minicircles.
Our second research direction is protein engineering. We evolve site-specific DNA recombinases of tyrosine type with new target specificity and analyze how individual amino acid substitutions influence the specificity switch. In this research we use popular site-specific recombinases Flp, Cre, as well as newly introduced TD and R. The tyrosine site-specific recombinases can mediate a full variety of DNA rearrangement reaction: integration and excision, inversion, translocation, and cassette replacement. These enzymes are particularly widespread in bacteria: over 1000 tyrosine site-specific recombinases are identified. All these enzymes have similar 3D fold but remarkably different target specificity. These properties make tyrosine recombinases an attractive model for changing target specificity of an enzyme and deciphering the functional role of their amino acid residues in target recognition. Moreover, the recombinase variants with modified target specificity could become useful gene editing and genome engineering tools.
Our third emerging research direction is aging. In these studies we want to understand how to reverse the harmful changes that cells acquire during the process aging. As a model organism, we use budding yeast since these yeast are able to reset their lifespan during spore formation. The details of the cell rejuvenation program are not yet known except that the transcription factor Ndt80, which can activate dozens of sporulation genes, is absolutely required. We want to analyze the role of the sporulation genes, expression of which is activated by Ndt80, in promoting the rejuvenation program in budding yeast and to determine the mechanism of the cell rejuvenation process.
Voziyanova E., Anderson R.P., Shah R., Li F., Voziyanov Y.
Efficient genome manipulation by variants of site-specific recombinases R and TD.
J. Mol. Biol., 2016
Shah R., Li F., Voziyanova E., Voziyanov Y.
Target-specific variants of Flp recombinase mediate genome engineering reactions in mammalian cells.
FEBS J., 2015
Voziyanova E., Malchin N., Anderson R.P., Yagil E., Kolot M., Voziyanov Y.
Efficient Flp-Int HK022 dual RMCE in mammalian cells.
Nucleic Acids Res., 2013
Anderson R.P., Voziyanova E., Voziyanov Y.
Flp and Cre expressed from Flp-2A-Cre and Flp-IRES-Cre transcription units mediate the highest level of dual recombinase-mediated cassette exchange.
Nucleic Acids Res., 2012
Shultz J.L., Voziyanova E., Konieczka J.H., Voziyanov Y.
A genome-wide analysis of FRT-like sequences in the human genome.
PLoS One, 2011
Malchin N., Molotsky T., Borovok I., Voziyanov Y., Kotlyar A.B., Yagil E., Kolot M.
High efficiency of a sequential recombinase-mediated cassette exchange reaction in Escherichia coli.
J. Mol. Microbiol. Biotechnol., 2010