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The frequency of required biomonitoring tests is specified in the NPDES
permit. Some facilities are required to test their effluent only annually.
Others may be required to test twice a year, while others may be required to test
quarterly, or even more often.
Upon completion of a test, the results are reported by the testing laboratory.
Although there is no particular specified format for these reports, a biomonitoring
report should include the following details.
 | General Information
 | Permit number |
 | Toxicity testing requirements of the permit |
 | Facility location |
 | Name of the testing laboratory |
|
 | Source of Effluent and Dilution Water
 | Sampling points |
 | Collection dates and times |
 | Sample collection method (i.e. grab sample or composite sample) |
 | Physical and chemical data |
|
 | Test Details
 | Type of biomonitoring test and treatments tested |
 | Date and time test started and ended |
 | Type and volume of test chambers |
 | Number of replicate chambers used with each treatment |
 | Number of organisms tested in each chamber |
 | Test temperature |
|
 | Test Organisms
 | Scientific name |
 | Age and source of test organisms |
|
 | Quality Assurance Details
 | Date and time of most recent reference toxicant test |
 | Reference toxicant used |
 | Results of reference toxicant test |
|
 | Biomonitoring Test Results
 | Raw data sheets showing daily records of survival and water quality information |
 | Tables showing required endpoints for the test (e.g. LC50, NOEC, LOEC etc.) |
 | Statistical Methods used to calculate endpoints |
|
 | Agency Forms |
Agency forms are pre-printed forms which are usually supplied with the permit.
When a test is completed, the agency forms are filled in with the tabulated results
of the test, and are submitted along with the biomonitoring report to the regulatory
agency.

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page last updated: 07/29/99
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